Isolation and Identification of Pathogenic Vibrio Species in Black Rockfish Sebastes schlegeli

Four pathogenic Vibrio species were isolated from three diseased black rockfish Sebastes schlegeli in Yantai, Shandong Province, China. The strains identified based on physiological and biochemical characteristics 16S rDNA sequencing named SF-2, SF-3, SF-5, SF-6, respectively. SF-2 was scophthalmi, SF-3 V. harveyi, SF-5 alginolyticus, SF-6 parahaemolyticus. This is the first time that scophthalmi rockfish. present research shows a potential pathogen. Detection of virulence genes using polymerase chain reaction showed carried FlaB; TcpA; ToxS. Tdh, Trh, Tlh, ToxR, Zot not detected. all had protease, gelatinase, lipase, lecithinase. They intermediately sensitive to erythromycin, whereas spectinomycin, cotrimoxazole chloramphenicol. resistant most antibiotics multidrug resistance obvious.